Tan, Zi Ni
(2016)
Physicochemical Properties And Prebiotic Potential Of Native, Resistant And Hcl-Resistant Starches From Sago (Metroxylon Sagu).
PhD thesis, Universiti Sains Malaysia.
Abstract
Kanji rintang jenis III (RS3) telah dihasilkan daripada sagu (Metroxylon sagu)
dan dinilai sifat fizikokimia and potensinya sebagai prebiotik. Sampel mengandungi
35.7% kanji rintang (dikenal sebagai sagu RS) telah dihasilkan apabila kanji sagu asli
diautoklaf dalam air suling pada suhu 121 °C selama 1 jam, dinyahcabang dengan 20
U pullulanase per g kanji pada 60 °C selama 24 jam dan seterusnya diautoklaf sekali
lagi pada 121 °C selama 1 jam sebelum disimpan pada 4 °C selama 24 jam.
Seterusnya, kandungan kanji rintang meningkat sehingga 63.8% (sampel dikenal
sebagai HCl-sagu RS) selepas sagu RS dihidrolisiskan dengan 0.5 M HCl pada suhu
60 °C. Granul sagu RS dan HCl-sagu RS menunjukkan corak pembelauan sinar X
jenis B, suhu puncak yang tinggi (143.7 °C and 146.5 ºC, masing-masing) dan
struktur permukaan yang tidak sekata dan kasar. Granul kanji sagu asli menunjukkan
corak pembelauan sina X jenis C, suhu puncak 74.6 °C dan permukaan yang sekata.
Keterlarutan dan kuasa pembengkakan sampel HCl-sagu RS ialah 14.9% dan 1.94
g/g, masing-masing, iaitu lebih rendah berbanding sagu RS (27.4% and 2.82 g/g,
masing-masing).
Resistant starch type III (RS3) was produced from sago (Metroxylon sagu)
and evaluated for its physicochemical properties and potential as a prebiotic. A
sample with 35.7% RS3 content (designated as sago RS) was produced when the
native sago starch was suspended in distilled water, gelatinized by autoclaving at
121 °C for 1 h, followed by debranching with 20 U pullulanase per g starch at 60 °C
for 24 h, autoclaved again at 121 °C for 1 h before storage at 4 °C for 24 h. RS3
content was further increased with the treatment of sago RS with 0.5 M HCl at 60 °C
(sample designated HCl-sago RS) to 63.8%. Granules of sago RS and HCl-sago RS
had B-type X-ray diffraction pattern, high peak temperatures (143.7 °C and 146.5 ºC,
respectively) and showed irregular and rough surface structure. While granules of
native sago starch had C-type diffraction pattern, peak temperature of 74.6 °C and
smooth granular surface. The solubility and the swelling power of HCl-sago RS
samples were 14.9% and 1.94 g/g, respectively, which were lower than that of sago
RS (27.4% and 2.82 g/g, respectively).
Actions (login required)
 |
View Item |
![[img]](http://eprints.usm.my/style/images/fileicons/application_pdf.png)
