Shahabudin, Nor Suhaida (2016) Optimization And Biocompatibility Of Alumina Foam Coated Scaffold For Bone Tissue Engineering Application. Masters thesis, Universiti Sains Malaysia.
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Abstract
Perancah busa alumina (AF) bersalut adalah salah satu bio sintetik yang mendapat perhatian daripada ahli-ahli sains bahan bagi mengatasi sifat lengai supaya interaksi ikatan tisu tulang dipertingkatkan. Kajian ini bertujuan untuk menghasilkan AF bersalut dengan kekuatan mampatan yang lebih tinggi daripada 2 MPa kepada 12 MPa, keliangan yang lebih tinggi daripada 70% kepada 99% dan saiz liang yang besar daripada 100 μm hingga 1000 μm yang diperlukan dalam aplikasi kejuruteraan tisu tulang. Faktor- faktor penting dalam teknik replikasi busa polimer (PFR) adalah jumlah liang (ppi) busa polimer (PU), nisbah komposisi pepejal kepada air ternyah-ion, peratusan pengikat dan kekerapan proses mecelup. Analisis DOE menggunakan rekabentuk pecahan 2k faktorial menunjukkan jumlah liang, nisbah komposisi dan bilangan mencelup adalah faktor terpenting mempengaruhi kekuatan mampatan perancah AF. Merujuk kepada rekabentuk kiub berpusat muka (FCC), nisbah komposisi 60/40, 20 ppi bilangan liang dan tiga kali proses mencelup adalah syarat yang memenuhi untuk perancah tulang manusia. Perancah AF dipertingkatkan dengan salutan hidroksiapatit (HACAF) dan hidroksiapatit-bentonit (HABCAF). Sampel-sample dinilai oleh analisis in-vitro iaitu rendaman di dalam simulasi cecair badan (SBF) dan ujian sitotosik (MTS assay). Analisis in-vitro menunjukkan permukaan perancah HACAF dan HABCAF mempunyai lapisan mendakan apatit dan kesan pertumbuhan sel yang positif. ________________________________________________________________________________________________________________________ Alumina foam (AF) coated scaffold is one of synthetics biomaterials that has received much attention to overcome inertness properties for enhanced bone tissue bonding interaction. This research aims to produce AF coated scaffold with compressive strength higher than 2 MPa to 12 MPa, porosity higher than 70% to 99% and pores size are larger than 100 μm to 1000 μm which required in bone tissue engineering application. The significant factors in polymer foam replication (PFR) techniques are number of pores (ppi) of polyurethane (PU) foam, the composition ratio of solid loading to deionized water, percentage of binder and number of dipping process. The DOE analysis using 2k fractional factorial designs shows that the number of pore, composition ratio and number of dipping are most significant factors effect on the compressive strength of AF scaffold. According to the face-centered cube (FCC) design, the condition satisfied for human bone scaffold is prepared at 60/40 composition ratio, 20 ppi numbers of pores and three times of dipping process. The AF scaffold was further improved by coated with hydroxyapatite (HACAF) and hydroxyapatite-bentonite (HABCAF). The samples were evaluated by in-vitro analysis which is immersed in simulated body fluid (SBF) solution and cytotoxicity by MTS assay. The in-vitro analysis showed the surface of HACAF and HABCAF scaffold have precipitations of apatite layer and positive effect on cell growth.
| Item Type: | Thesis (Masters) |
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| Additional Information: | Full text is available at http://irplus.eng.usm.my:8080/ir_plus/institutionalPublicationPublicView.action?institutionalItemId=3070 |
| Subjects: | T Technology T Technology > TN Mining Engineering. Metallurgy > TN263-271 Mineral deposits. Metallic ore deposits. Prospecting |
| Divisions: | Kampus Kejuruteraan (Engineering Campus) > Pusat Pengajian Kejuruteraan Bahan & Sumber Mineral (School of Material & Mineral Resource Engineering) > Thesis |
| Depositing User: | Mr Mohd Jasnizam Mohd Salleh |
| Date Deposited: | 29 Aug 2018 07:02 |
| Last Modified: | 29 Aug 2018 07:37 |
| URI: | http://eprints.usm.my/id/eprint/41569 |
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