Tai, Wan Yi
(2016)
Enhancement Of Cellulase-Poor
Xylanase Production By Native Fungi
Via Solid State Fermentation Process.
Masters thesis, Perpustakaan Hamzah Sendut.
Abstract
High cost of cellulases remains the most significant barrier to the economical
production of bio-ethanol from lignocellulosic biomass (LB). The present study aims at
developing a local cellulolytic fungal strain with enhanced cellulolytic ability through
random mutagenesis coupled with the feasibility of solid-state fermentation (SSF) by
utilizing oil palm frond (OPF) as the substrate. Out of 95 wild isolates isolated from
agricultural sites and tested both qualitatively and quantitatively, a native filamentous
fungal strain designated DWA8 was found to be the top enzymatic secretor. During
qualitative screening, 38 isolates were found to produce distinctive halo but only 23
were chosen to proceed with quantitative screening. For quantitative enzyme analysis,
enzyme supernatant was extracted from the SSF process which was conducted using
IxlO6 spore/mL inoculated onto 5 g of ground OPF, incubated at room temperature for 7
days. DWA8 was found to be the best candidate for further studies, as it produced the
highest amount of fpase and xylanase with a considerably high amount of cmcase, which
Aspergillus niger (Genebank accession no. KP299287) based on colony morphology and
the best cultivation period where the cmcase, fpase and xylanase activities of A. niger
DWA8 were 1.13 U/g, 2.55 U/g and 2.38 U/g respectively.
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