Noor Izani, Noor Jamil
(2012)
Antimicrobial properties of kelulut (Trigona Spp) honey.
Other.
Pusat Pengajian Sains Perubatan.
(Submitted)
Abstract
The antibacterial activities ofKelulut (Trigona spp) honey obtained from the local honey
supplier in Kota Bharu, Kelantan was investigated. "Kelulut" honey was tested for its
antimicrobial activity by disc diffusion and well diffusion assays and the minimum
inhibitory concentration (MIC) was determined using the standard assay procedures. In
terms of physicochemical properties, it has a dark amber appearance with a pH of 2.50,
specific gravity of 1.26, moisture content was 20.6%, dry matter content was 79.4% and
lipid content of 1% (w/w). The "Kelulut" honey exhibited significant antibacterial
activities towards the Gram positive and Gram negative bacteria at the concentration of
20-80 (%, v/v) and the neat hJney (100%, v/v) produced the best antibacterial activity
compared to other dilutions. In the minimum inhibitory concentration determination, all
the strains were most sensitive in the range of (35-70J.!g/ml). The honey organic solvent
extract did not exhibit significance antibacterial effect. However, the "Kelulut" honey did
not show antifungal activity even at very high concentration. The "kelulut" honey was
found to have low anti-oxidant activity.
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