Sezale, Nur Syahirah (2018) Immobilization Of β-Galactosidase Onto Activated Chitosan Support By Using Covalent Binding Method. Project Report. Universiti Sains Malaysia, Pusat Pengajian Kejuruteraan Kimia. (Submitted)
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Abstract
The performance of immobilized β-galactosidase (β-Gal) onto chitosan support was studied in a batch process. The chitosan support was activated by using chemical agent which was glutaraldehyde solution. The main objective of this research was to investigate the suitable conditions in preparation of β-Gal immobilization process that will produce greater β-Gal activity. During the preparation of immobilized β-Gal, the optimum conditions were obtained from Response Surface Methodology (RSM). The optimum conditions were found to be 2% and 10 mg/ml for glutaraldehyde and β-Gal concentrations, respectively, which has resulted in 65.52% for activity yield meanwhile for immobilization yield, is 94.57%. The experimental results for investigation of effect of temperature on β-Gal activity, found that the optimum temperature at ℃ was obtained after β-Gal immobilization. However, it is so interesting that the immobilized β-Gal showed much better operational stability referring to a broader thermal range as compared to free β-Gal. The activity of immobilized β-Gal increased at about more than 17% of its initial activity, meanwhile the activity of free β-Gal reduced at less than 15%. Higher stability of β-Gal will lead to a better reusability. The immobilized β-Gal can be reused for six cycles and retained approximately 50% of its initial activity.
| Item Type: | Monograph (Project Report) |
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| Subjects: | T Technology T Technology > TP Chemical Technology |
| Divisions: | Kampus Kejuruteraan (Engineering Campus) > Pusat Pengajian Kejuruteraan Kimia (School of Chemical Engineering) > Monograph |
| Depositing User: | Mr Engku Shahidil Engku Ab Rahman |
| Date Deposited: | 28 Jul 2022 09:26 |
| Last Modified: | 28 Jul 2022 09:26 |
| URI: | http://eprints.usm.my/id/eprint/53696 |
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