Ezzreen Zafhrina Mohamad Redza, Nur
(2020)
Fermentation And Purification Of Lipase
By Burkholderia Metallica
Using Column Reactor.
Project Report.
Pusat Pengajian Teknologi Industri.
(Submitted)
Abstract
In this work, fermentation was conducted for the feedstock and purification of lipase by
Burkholderia metallica using a column reactor. The targeted parameter for this research such as
salt stability, types and concentrations of polyethylene glycol(PEG) and salt, pH of extraction
solution and flowrate ml/s to see the performance of extractive purification system. Aqueous
impregnated resin system(AIRS) is applied using porous glass beads grafted with polyethylene
glycol (PEG). An evaluation studies on AIRS were performed with various molecular weight of
polyethylene glycol (PEG) (PEG 2,000, PEG 4,000, PEG 6,000) and different type of salts
(sodium citrate, potassium citrate and sodium acetate) as phase component. Liquid extractant of
PEG was immobilized on the inert support (porous glass beads) and correspond to the aqueous
state phase to surmount the drawback of aqueous two phase extraction (ATPE). The technique
combines liquid-liquid extraction with ease if operation of column chromatography.
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