Kok, Boon Hui
(2020)
Production Of Novel Recombinant Anti-PFHRP2 VNAR-G1 Protein Using Escherichia
COLI BL21(DE3) Expression System.
Project Report.
Pusat Pengajian Teknologi Industri.
(Submitted)
Abstract
Malaria rapid diagnostic tests (RDTs) act as important antibody-based immunoassays
for prompt malaria diagnosis. Conventional monoclonal antibodies (mAbs) are
widely used in RDTs but it can be easily degraded at high ambient temperatures.
Hence, the shark VNARS might be good alternatives to mAbs due to its higher thermal
stability and binding affinity with antigens. In this study, the recombinant antiPfHRP2 VNAR-G1 protein was produced in E. coli expression system through various
steps such as recombinant cell isolation, PCR, agarose gel electrophoresis, plasmid
extraction, transformation and protein expression.
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