Purification And Characterization Of Protease From Artocarpus Integer Leaf

Zulfigar,, Siti Balqis (2012) Purification And Characterization Of Protease From Artocarpus Integer Leaf. Masters thesis, Universiti Sains Malaysia.

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Abstract

The presence of a protease in Artocarpus integer leaves, which can be used as a meat tenderiser, was verified by the presence of a band at 69 kDa, using caseinolytic zymography and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). Purification by temperature-phase partitioning with 6 % (v/v) Triton X-114, 44 % (w/v) ammonium sulphate precipitation and gel filtration chromatography yielded a preparation with a 12-fold increase in enzyme purity and a final specific activity of 76.67 U/mg. The purified protease was maximally active at 40ºC and at pH 10.0.

Item Type:	Thesis (Masters)
Subjects:	T Technology > T Technology (General) > T1-995 Technology(General)
Divisions:	Pusat Pengajian Teknologi Industri (School of Industrial Technology) > Thesis
Depositing User:	ASM Ab Shukor Mustapa
Date Deposited:	05 Jul 2019 08:15
Last Modified:	05 Jul 2019 08:15
URI:	http://eprints.usm.my/id/eprint/44895

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