Abd Rashid, Norlinda and Hapidin, Hermizi and Abdullah, Hasmah and Ismail, Zalina and Long, Idris
(2017)
Nicotine-prevented learning and memory impairment in REM
sleep-deprived rat is modulated by DREAM protein in the
hippocampus.
brain and behavior.
ISSN 2162-3279
Abstract
Introduction: REM sleep deprivation is associated with impairment in learning and
memory, and nicotine treatment has been shown to attenuate this effect. Recent studies
have demonstrated the importance of DREAM protein in learning and memory
processes. This study investigates the association of DREAM protein in REM sleep-deprived
rats hippocampus upon nicotine treatment.
Methods: Male Sprague Dawley rats were subjected to normal condition, REM sleep deprivation
and control wide platform condition for 72 hr. During this procedure, saline or
nicotine (1 mg/kg) was given subcutaneously twice a day. Then, Morris water maze (MWM)
test was used to assess learning and memory performance of the rats. The rats were sacrificed
and the brain was harvested for immunohistochemistry and Western blot analysis.
Results: MWM test found that REM sleep deprivation significantly impaired learning
and memory performance without defect in locomotor function associated with a significant
increase in hippocampus DREAM protein expression in CA1, CA2, CA3, and
DG regions and the mean relative level of DREAM protein compared to other experimental
groups. Treatment with acute nicotine significantly prevented these effects
and decreased expression of DREAM protein in all the hippocampus regions but only
slightly reduce the mean relative level of DREAM protein.
Conclusion: This study suggests that changes in DREAM protein expression in CA1,
CA2, CA3, and DG regions of rat’s hippocampus and mean relative level of DREAM
protein may involve in the mechanism of nicotine treatment-prevented
REM sleep
deprivation-induced
learning and memory impairment in rats
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