Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis

Mohamad, Suharni (2009) Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis. PhD thesis, Universiti Sains Malaysia.

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Abstract

In this study, recombinant T. canis DNA which encode for rTES-26, rTES-32 and rTES-120 were produced by cloning of open-reading frames (ORF) of the respective genes via reverse-transcriptase-PCR (RT-PCR) using mRNA extracted from a culture of T. canis second stage larvae into PCR2.1 TOPO vector. Dalam kajian ini, DNA rekombinan T. canis yang mengkodkan antigen rekombinan rTES-26, rTES-32 dan rTES-120 dihasilkan dengan mengklonkan kerangka bacaan terbuka gen masing-masing melalui kaedah “reverse-transcriptase-PCR” (RT-PCR) menggunakan mRNA yang diekstrak daripada kultur larva T. canis peringkat kedua ke dalam vektor PCR2.1 TOPO.

Item Type: Thesis (PhD)
Subjects: R Medicine > R Medicine (General) > R5-130.5 General works
Divisions: Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Perubatan (School of Medical Sciences) > Thesis
Depositing User: Administrator Automasi
Date Deposited: 03 Jun 2015 06:35
Last Modified: 15 May 2017 04:43
URI: http://eprints.usm.my/id/eprint/28927

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