Xu, Yaping (2025) Therapeutic Effects Of Human Pericardial Fluid Cells In Doxorubicin-Induced Heart Failure Rat Model Via Intrapericardial Injection. PhD thesis, Perpustakaan Hamzah Sendut.
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Abstract
Heart failure (hf) is the leading cause of mortality worldwide. Cell therapy has emerged as an alternative strategy for treating hf. This study aimed to isolate and characterize human pericardial fluid-derived cells (hpfcs) and investigate the cell engraftment, therapeutic effects, and mechanisms of action after intrapericardially administered into a doxorubicin-induced hf rat model. Hpfcs were isolated from the pericardial fluid of five hf patients who underwent heart transplantation and cultured. The cultured hpfcs showed the expression of stem cell markers nanog+/sca-1+ (94.0% ± 1.8%), c-kit+/nanog+ (30% ± 4.0%), c-kit+/sca-1+ (16.6% ± 4.4%), and cd90+/cd105+ (76.8% ± 15.5%) at passage 3. Additionally, hemopoietic markers cd45+cd90+ (35.1% ± 19.7%) and cd34+cd73+ (13.9% ± 6.9%) were also expressed. These hpfcs were also able to differentiate into adipocytes (70.6% ± 1.2%), osteoblasts (29.3% ± 3.3%), and cardiomyocytes (81% ± 1.6%) in vitro. Doxorubicin (dox) was used to induce hf in rats for 6 weeks (2.0 mg/ml). Hf characteristics were confirmed at week 4 by examining the changes in cardiac function, fibrosis, and the expression of inflammatory cytokines and angiogenic factors prior to administering hpfcs (2.5 × 105 cells/heart) intrapericardially at passage 2. The same parameters were re-assessed for changes after 1 and 4 weeks of cell administration
| Item Type: | Thesis (PhD) |
|---|---|
| Subjects: | R Medicine > R Medicine (General) > R5-920 Medicine (General) |
| Divisions: | Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute (AMDI)) > Thesis |
| Depositing User: | Mr Hasmizar Mansor |
| Date Deposited: | 07 May 2026 02:24 |
| Last Modified: | 07 May 2026 02:24 |
| URI: | http://eprints.usm.my/id/eprint/64105 |
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