Rama, Ballouze (2021) Synthesis, Characterization, And Cytotoxicity Evaluation Of Magnesium-doped Biphasic Calcium Phosphate (Mg-bcp) Powders. Masters thesis, Universiti Sains Malaysia.
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Abstract
Recently, bone and dental surgeries are becoming remarkably prevalent, and the natural bone grafts shortage in this area is evident. Thus, the need for synthetic bone graft substitutes is increasing. Synthetic calcium phosphates (CaPs) are ideal, resorbable, and biocompatible biomaterials that are almost identical to the mineral part of the bone and tooth enamel, especially when they are doped with different ions. Since magnesium ion (Mg) performs significant functions throughout the human body, Mg-doped biphasic calcium phosphate (Mg-BCP) gets special attention among other CaPs. Besides biocompatibility, osteoconductive, and osteoinductive properties, Mg-BCP has better mechanical properties. In this project, Mg-BCP powder with HA:βTCP ratio equals 60:40, and Mg substitution of 1 wt% was synthesized using the chemical wet precipitation method and characterized via XRD, SEM-EDX, and FT-IR techniques. After that, several doses (1 to 400 μg/mL) of MgBCP were co-cultured with hFOB 1.19 cells for 24, 48, and 72 h for in vitro cytotoxicity evaluation and microscopic observation. Additionally, alkaline phosphatase (ALP) activity of hFOB 1.19 cells was investigated after 72 h co-culture with 50 and 200 μg/mL doses of Mg-BCP. For all the experiments, pristine BCP was included for comparison purposes. As a result, Mg-BCP powder with the desired phase composition ratio (HA:βTCP = 60:40, 1 wt% Mg) was successfully synthesized as confirmed by XRD, FT-IR, and SEM-EDX analysis. Cytotoxicity evaluation indicated that Mg-BCP did cause a certain reduction in cell viability. However, the reduction was not severe even when hFOB 1.19 cells were co-cultured with the highest dose of the synthesized powder (400μg/mL) for the longest incubation period (72 h); the cell viability was above 83% with no changes in the cell morphology. Interestingly, the Mg-BCP powder was less toxic than BCP. Moreover, increasing the Mg-BCP dose significantly enhanced the ALP activity in hFOB 1.19 cells compared to BCP and untreated cells. In conclusion, the Mg-BCP powder with a phase composition ratio equals 60:40 and 1 wt% Mg is a promising bone graft substitute.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | R Medicine |
| Divisions: | Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute (AMDI)) > Thesis |
| Depositing User: | NOR HASHIMY BIN ABDULLAH |
| Date Deposited: | 05 May 2026 03:53 |
| Last Modified: | 05 May 2026 03:53 |
| URI: | http://eprints.usm.my/id/eprint/64073 |
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