Baharuddin, Mohd Hazwan (2020) A study on fusarium isolation, its molecular identification, clinical manifestations and risk factors. Masters thesis, Universiti Sains Malaysia.
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Abstract
Fusarium species are ubiquitous in soil and plant debris. Fusarium species are well recognized as plant pathogens and have emerged as human pathogens affecting both immunocompetent and immunocompromised hosts since recent years. Conventional methods of Fusarium identification are inadequate as it only identifies Fusarium to genus level. Molecular methods by nucleotide sequences are more accurate and consistent for species identification. We reviewed 87 medical records of patients for whom fungal culture was performed on various clinical specimens in Hospital Sultanah Nur Zahirah, Terengganu and Hospital Universiti Sains Malaysia, Kelantan from 2017 until 2019. Out of 87 cases, 43 cases were positive for Fusarium and 44 cases were non-Fusarium cases. We identified the proportion of Fusarium based on species, clinical manifestation, and risk factors from clinical isolates. Twenty four available clinical isolates were specifically identified by sequencing the translation elongation factor 1-alpha (TEF-1) gene. Our results showed that keratitis (n=17, 39.5%) and onychomycosis (n=16, 37.2%) were the most common type of fusariosis, followed by fungaemia (n=7, 16.3%). Forearm skin infection, fungal pneumonia, and fungal maxillary sinusitis were presented in one case each. Based on TEF-1 sequencing, 22 of 24 isolates belong to Fusarium solani species complex (FSSC), n=22; which comprised Fusarium solani (n=10), Fusarium proliferatum (n=7), and Fusarium keratoplasticum (n=5). Another two were Fusarium pseudocircinatum (n=1) which belongs to Fusarium fujikuroi species complex (FFSC), and Fusarium oxysporum (n=1) which is a member in Fusarium oxysporum species complex (FOSC). Fusarium infection was more common in males (n=29, 67.4%), and the mean age was 51 years old. Important risk factors for Fusarium infection, including diabetes mellitus, malignancies, neutropenia, eyes trauma, and embedded foreign body, were analysed but none were statistically significant
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Fusarium,risk factors |
| Subjects: | Q Science > QR Microbiology R Medicine |
| Divisions: | Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Perubatan (School of Medical Sciences) > Thesis |
| Depositing User: | MUHAMMAD AKIF AIMAN AB SHUKOR |
| Date Deposited: | 08 Apr 2026 04:37 |
| Last Modified: | 03 Jun 2026 07:31 |
| URI: | http://eprints.usm.my/id/eprint/63857 |
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