Zainoddin, Haziq Ahmad Hazwan (2023) The Effects Of Nannochloropsis Sp. Methanolic Extract On Prevention Of Dna Damage In L929 Cells. Masters thesis, Universiti Sains Malaysia.
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Abstract
Nannochloropsis and Spirulina are some of microalgae species that have been found to have a wide range of biological activities such as antioxidant and anti-DNA damage properties. Unfortunately, there is still lack of study on the effect of both microalgae especially Nannochloropsis species. Hence, this study was carried out to determine the effect of Nannochloropsis sp. extract on prevention of DNA damage in L929 cell line. The phytochemicals of both microalgae were extracted using 100% methanol. The extractions were then screened with HPLC to detect the presence of targeted compounds. HPLC analysis revealed that Nannochloropsis sp. methanolic extracts had caffeic acid, p-Coumaric acid, naringenin and hesperetin while Spirulina sp. methanolic extract had caffeic acid, quercetin and naringenin. The extracts were then tested for Total Phenolic Content (TPC) and Total Flavonoid Content (TFC). Results revealed that the extracts for both TPC and TFC for Nannochloropsis sp. were higher than Spirulina sp. with 58.43 mg GAE/g and 19.64 mg GAE/g, and 79.87 mg QE/g and 11.19 mg QE/g respectively. The study was then proceeded by evaluating the antioxidant activities of the microalgae extracts using DPPH-HPLC analysis. Nannochloropsis sp. methanolic extract exhibit greater percentage of DPPH inhibition compared to Spirulina sp. extract. A negative correlation existed between TPC and TFC with the EC50 of DPPH antioxidant inhibition activity. In this study, Spirulina sp. and Nannochloropsis sp. showed moderate toxicity toward L929 cell lines with IC50 1.701 mg/mL and 1.134 mg/mL respectively. In DNA damage analysis with Nannochloropsis sp., the result revealed that the methanolic extract are able to prevent and reduce DNA damage activity. Thus, microalgae can be proposed as one of alternative form in medical and pharmaceutical industry.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | R Medicine R Medicine > R Medicine (General) |
| Divisions: | Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute (AMDI)) > Thesis |
| Depositing User: | MUHAMAD AMIN BIN AZMI |
| Date Deposited: | 05 Mar 2026 03:15 |
| Last Modified: | 05 Mar 2026 03:18 |
| URI: | http://eprints.usm.my/id/eprint/63717 |
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