Ruslan, Muhammad Azim Haikal (2024) The differentiation effect of atorvastatin and rosuvastatin on mitochondrial function in l6 muscle cells. Project Report. Universiti Sains Malaysia. (Submitted)
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Abstract
Statins, widely prescribed for managing hyperlipidemia and reducing cardiovascular risks, are associated with adverse effects on skeletal muscle cells, potentially due to mitochondrial dysfunction. The effects of statins on muscle cells vary due to their solubility properties. This study compares the differential effects of atorvastatin (lipophilic statin) and rosuvastatin (hydrophilic statin) on the viability (MTT assay) and the mitochondrial function in L6 skeletal muscle cells. Using MTT assays, atorvastatin exhibited greater cytotoxicity with an IC50 value of 160.0 μM (95% CI: 136.6–201.7) compared to rosuvastatin (IC50 > 200 μM). Lactate dehydrogenase (LDH) assays assessed mitochondrial function, with cells treated with atorvastatin and rosuvastatin exhibiting a comparable effect on LDH retention with α-cyano-4-hydroxycinnamate (CHC), a non-specific MCT1 inhibitor. Specifically, the LDH activity compared to non-treated (NT) cells was significantly higher in CHC-treated cells (p < 0.0001), atorvastatin-treated cells (p < 0.0001), and rosuvastatin-treated cells (p < 0.0001). LDH activity was also found to be affected by cells passage number resulting in significant mean differences in the LDH levels (p < 0.05 and p < 0.01, respectively). These findings suggest that lipophilic statin exerts a more pronounced effect on L6 cells viability than hydrophilic statin; however, both types of statins affect mitochondrial function to a similar degree with MCT1 modulation. The study highlights the potential consequences of both statins modulating MCT1 in causing an effect on mitochondrial function
| Item Type: | Monograph (Project Report) |
|---|---|
| Uncontrolled Keywords: | rosuvastatin |
| Subjects: | R Medicine R Medicine > RA Public aspects of medicine |
| Divisions: | Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Perubatan (School of Medical Sciences) > Monograph |
| Depositing User: | MUHAMMAD AKIF AIMAN AB SHUKOR |
| Date Deposited: | 09 Dec 2025 03:52 |
| Last Modified: | 25 Feb 2026 02:34 |
| URI: | http://eprints.usm.my/id/eprint/63382 |
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