Ayoona, Daniah Fouad Abdulameer
(2024)
Phytochemical Characterization And Wound Healing Property Of Euphorbia Hirta L.
PhD thesis, Perpustakaan Hamzah Sendut.
Abstract
Euphorbia hirta l, an annual plant that belongs to the euphorbiaceae family,
is a common traditional remedy for several diseases. Currently, there are few
investigations on this plant as a treatment for wound healing, particularly the bioactive
compounds and mechanism of action. Therefore, this study focused on isolating the
main active compounds associated with the wound-healing activity of e. Hirta. The
aerial parts of the plant were collected from the northern part of malaysia and iraq and
then were extracted with solvents of different polarities: hexane, chloroform,
methanol, and water. All extracts were screened for total phenolic and flavonoid
contents and antioxidant and anti-inflammatory activities. Additionally, all extracts
were assessed for potential wound healing activity by measuring the migration and cell
viability of human fibroblast cells, hs27. Then the methanol extracts were further
fractionated to identify the active compounds. The structure elucidation of isolated
compounds was confirmed using liquid chromatography-mass spectrometry (lcms/
ms), nuclear magnetic resonance (nmr), and high-performance liquid
chromatography (hplc). Subsequently, molecular docking was performed on the
isolated compounds to analyse their binding interactions with the glycogen synthase
kinase-3β (gsk3b) and casein kinase 1 (ck1) proteins. The level of mrna
expression of four cytokines (il1b, il6, tnf, and col1a1) after treating human
fibroblasts with different compounds were assayed. The e. Hirta extract yielded a
different type of compounds: kaempferol,
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