Shuraish, Paveethra
(2024)
Synthesis Of Novel Carbon Dots From
Polyalthia Longifolia Leaf For In Vitro
Cytotoxicity Against Hela Cells.
Masters thesis, Universiti Sains Malaysia.
Abstract
Carbon dots (CDs), known for their outstanding fluorescence properties, have
found extensive applications in medicine and pharmacy owing to their
biocompatibility and nano-sized attributes. However, the potential cytotoxic effects of
CDs derived from Polyalthia longifolia rich with various pharmacological activity
remain underexplored. To address this gap, the current study synthesized,
characterized, and investigated the toxicity against RAW264.7 cells, as well as photoinduced
cytotoxicity against HeLa cells of the CDs derived from P. longifolia. Various
methods, including transmission electron microscopy (TEM), X-ray diffraction
(XRD), ultraviolet-visible (UV-vis) spectroscopy, zeta potential, and Fouriertransform
infrared spectroscopy (FTIR), were employed to characterize the CDs.
Additionally, the toxicity of the synthesized CDs was evaluated against RAW 264.7
cells, and the photo-induced cytotoxicity was assessed against HeLa cells under white
light. Moreover, the impact of photo activation on ROS generation in HeLa cells also
investigated. The synthesized spherical black spheres exhibited an average size of 6.29
± 0.31 nm (TEM), consisting of both crystalline and amorphous phases of carbon
(XRD), and a negative zeta potential of -20.7 ± 2.01 mV. The synthesized CDs emitted
blue fluorescence when exposed to UV light at 365 nm and exhibited a prominent UVvisible
absorption peak in the UV region. FTIR analysis revealed the existence of
diverse functional groups on the CDs. The toxicity evaluation of synthesized CDs
indicated low toxicity, promoting RAW264.7 cell viability within the 500–8000 μg
range (P<0.05).
Actions (login required)
 |
View Item |
