Periayah, Mercy Halleluyah
(2015)
Understanding Platelet Thrombogenicity Cascade
Of The Biodegradable Chitosan Derivatives
In Von Willebrand Disease In Vitro.
PhD thesis, Universiti Sains Malaysia.
Abstract
Chitosan has become one of the most promising local hemostatic agents because it is
of particular interest as it functions independently on platelets and normal clotting
mechanisms. The present study was designed with the aim to test the ability of the
mechanisms of blood platelets towards the action of biodegradable chitosan in
normal donors and von Willebrand disease (vWD) patients in vitro. This work
determined the underlying mechanism of chitosan-induced platelet thrombogenicity
and comprises experimental tests such as degradation ability; platelet: adhesion,
activation: aggregation; coagulation analysis and hemostatic mediators: von
Willebrand Factor (vWF), Factor 8 (FVIII), Thromboxane A2 (TXA2), P2Y12,
glycoprotein IIbIIIa (GpIIbIIIa), Transforming Growth Factor- Beta 1 (TGF-β1) and
Platelet Derived Growth Factor-AB (PDGF-AB). Comparative studies have been
conducted to measure the hemostatic capacity of biodegradable 7% N,OCarboxymethylchitosan
(NO-CMC) (with 0.45 mL collagen), 8% NO-CMC, Oligochitosan
(O-C) and O-C 53.
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