Yusoff Azudin, Nurhazwani
(2014)
Modeling And Optimization Of Solvent-Free Isoamyl Acetate Production Via Enzymatic Esterification.
Masters thesis, Perpustakaan Hamzah Sendut.
Abstract
Isoamyl acetate is an organic compound listed under the esterfamily, and used as
flavor additive especially in food industries. Studies on esterification processes
begin with traditional extraction of ester from plants,followed by chemical synthesis
route. Nowadays, the esterification process has shifted to biocatalytic flavor due to
consumer's inclination towards natural compared to chemical resources. Thisstudy
was carried out to examine the reaction synthesis between acetic anhydride and
isoamyl alcohol in the presence of Candida antarctica Lipase-B (CALB) as a
catalyst in solvent-free system (SFS). Results show that two reactions took place: (i)
between acetic anhydride and isoamyl alcohol, and (ii) between acetic acid and
isoamyl alcohol. Effect of different reaction parameters on the final yield of isoamyl
acetate, and optimization of process parametersusing a statistical tool were also
investigatedwithresponse surface methodology (RSM).
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