Asohan, Anusha Wei
(2023)
Fabrication Of 3d Double-network Cnc/alg/pegda Printed Constructs Seeded With C2c12 Myoblasts For Bioprinting Applications.
Masters thesis, Universiti Sains Malaysia.
Abstract
3D bioprinting offers a promising alternative for articular cartilage repair and
regeneration using a layer-by-layer predetermined movement from a computer aided
(CAD) software. In this project, a bioink for bioprinting applications was formulated
using cellulose nanocrystals (CNCs), alginate (Alg), and poly(ethylene glycol)
diacrylate (PEGDA). Firstly, different formulations of CNC/Alg/PEGDA were
formulated using a double network crosslinking approach where the hydrogel was first
ionically crosslinked with Ca2+ ions and subsequently photo-crosslinked under UV
irradiation at 365nm post-extrusion. The rheological properties, swelling behaviour,
and mechanical properties of the formulations were assessed to determine the optimal
formulation for bioprinting. Then, the printability of the selected CNC/Alg/PEGDA
formulation was evaluated using an extrusion based bioprinter. Next, the
biocompatibility of the selected CNC/Alg/PEGDA formulation was determined using
C2C12 myoblasts. The cells were seeded into the hydrogel, followed by the bioprinting
process. The viability of cells was assessed using Live/Dead assay for a period of 14
to 16 days. The internal structure and porosity of the bioprinted construct with and
without cells was analysed using scanning electron microscopy (SEM). Based on the
preliminary results, Formulation 8 (F8) with the concentration of 4% CNC, 4% Alg
and 40% PEGDA demonstrated shear-thinning behaviour with power-law inde
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