Umam, Nada Itorul
(2023)
Ultraviolet Assisted Autolysis Of Sea Cucumber (Stichopus Horrens) Body Wall For The Enhancement Of Sulfated Polysaccharides Release.
Masters thesis, Universiti Sains Malaysia.
Abstract
Sea cucumbers (Stichopus horrens) contain important bioactive compounds
called sulfated polysaccharides (SP), which can be extracted by autolysis process
utilizing its endogenous enzyme. However, this process is slow and not efficient. The
aim of this study was to evaluate the mechanism of UV assisted autolysis in enhancing
the release of SP from sea cucumber body wall. In phase I, the effects of UV dosage
and pH on endogenous proteases activity, biochemical, and structural changes of
SHBW during autolysis were evaluated. Frozen SHBW was homogenized with and
without buffer (pH 6, 7, 8) and exposed to low (10,800 J/m2) and high (324,000 J/m2)
UV dosage prior to autolysis at 50 °C for 4 and 8 hours. The endogenous protease
activity and respective biochemical changes were evaluated from the soluble fraction
while the insoluble fraction of SHBW was evaluated for secondary protein structural
changes and thermal stability using Fourier-transform Infrared Spectroscopy (FTIR),
Differential Scanning Calorimetry (DSC), and Thermogravimetry Analysis (TGA).
Increase in UV dosage by 30x, increased proteolytic activity by 4.5-fold after 4 hours
autolysis, resulting in increased soluble protein, hydroxyproline, and
glycosaminoglycan (GAG) content by 5, 4, and 3 folds, respectively. Although sample
without buffer showed similar endogenous protease activity with control sample, the
total soluble protein, hydroxyproline, and GAG were higher by 4, 0.1, and 2.5 folds,
respectively.
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