Che Ani, Mohd Suhaimi
(2022)
The Accumulation Of Chloroplast Small Rnas In Chlamydomonas Reinhardtii During Nitrogen Deprivation.
PhD thesis, Perpustakaan Hamzah Sendut.
Abstract
The effects of the availability of nitrogen to chloroplast bioenergetics are
beneficial and very significant that need to be explored. Small RNAs have been
identified from the genome of chloroplasts, yet limited information exists on the
response of small RNAs to chloroplasts during nitrogen deprivation. In this study,
the participation of small RNAs is explored in order to gain a better understanding of
their roles in algae's regulatory mechanisms during stress adaptation. A comparative
transcriptomic survey was conducted to find chloroplast small RNAs that respond to
nitrogen deprivation in the microalgae Chlamydomonas reinhardtii. Growth in
nitrogen-deprived medium revealed 22 significantly over-accumulated and 18 lessaccumulated
chloroplast small RNAs. The genomic locations of these small RNAs
varied covering regions that were either untranslated, or coded in the sense or
antisense direction, or the ends of the tRNA and rRNA genes.
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