Yu-Chen, Ng
(2022)
Molecular discrimination of bovine origin from processed canned products using PCR amplification of cytochrome B gene.
Masters thesis, Universiti Sains Malaysia.
Abstract
The molecular method become the preferred technique used for the identification of animal species from processed meat products. PCR-based species-specific primers offer an advantage by targeting a specific species using a certain gene. Therefore, this study is aimed to identify beef meat content from processed canned products around Kubang Kerian, Kelantan. DNA extraction of 12 canned products and one fresh raw beef meat was subjected to a DNeasy mericon food kit from Qiagen and quantification was performed using a Nanodrop spectrophotometer. PCR amplification using species-specific primer by targeting a mitochondrial DNA cytochrome b gene was amplified 120 bp of the target region. Success amplification was seen in the positive control sample (raw beef meat) and two canned products. Out of 12 processed canned products, 83.33% failed to detect or no DNA band was seen at 120 bp. This could be influenced by the manufacturing process for the canned products which involves high temperature and pressure treatment, hence affecting the genomic DNA to become degraded and/or possibly a very low amount of DNA was obtained from DNA extraction. In summary, the use of mitochondrial DNA markers specifically the cytochrome b gene has increased the possibility of the amplification of the targeted gene from processed canned products due to high copy number.
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