Lansayan, Jalviana
(2022)
Genetic polymorphisms of
proinflammatory (IL-1β) cytokines in
Malay male drug abusers with
Hepatitis C infection.
Masters thesis, Universiti Sains Malaysia.
Abstract
The Hepatitis C virus (HCV) infection frequently results in liver conditions
such fibrosis, cirrhosis, and hepatocellular carcinoma, therefore, it poses a serious risk
to the general public health. Furthermore, the incidence of HCV infection transmission
is concerning among drug abusers. The intercellular mediators of inflammation,
cytokines, are byproduct in viral control and the liver damage brought on by hepatitis
C virus infection. IL-1β, particularly, is a proinflammatory cytokine that is secreted
during the innate and adaptive immune response that is pivotal in chronic
inflammatory diseases. Studies have reported the association of this cytokine and
susceptibility of chronic HCV infection. Therefore, the purpose of the study was to
investigate the role of IL-1β polymorphisms (rs16944 and rs1143634) in regard to
chronic hepatitis C infection susceptibility. A total of 48 Malay male drug abuser were
involved in this case-control retrospective study. The genomic DNA was extracted
from whole blood sample then analyzed through polymerase chain reaction-restriction
fragment length polymorphism (PCR-RFLP) for the IL-1β rs16944) and IL-1β
(rs1143634) polymorphisms. The analysis of IL-1β (rs1143634) showed that CC
genotype was common in both case and control group. However, no statistical
significance was achieved. This research concluded that there was no association
between the polymorphisms (rs16944 and rs1143634) and susceptibility to hepatitis C
infection among Malay male drug abuser.
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