Alsocoty, Ameera Kamal Khaleel
(2022)
Assessment of the protective role of green tea Extract against the ciprofloxacin induced toxicity on Mandibular condylar cartilage of wistar rats.
PhD thesis, Universiti Sains Malaysia.
Abstract
The head of the mandibular condyle is covered with mandibular condylar cartilage
which acts as the center of growth in the craniofacial complex. Ciprofloxacin is a
fluoroquinolone which is important for treating a life-threatening bacterial infection. It
has a chondrotoxic effect in growing condylar cartilage. Green tea has attracted a
significant attention for its multiple health benefits, and the previous researches uncover
the importance of dietary choices for prevention of the chondrotoxic effect of
ciprofloxacin. The objective of the present study was to investigate the preventive
potential of green tea extract on the mandibular chondrotoxicity induced by
ciprofloxacin in juvenile male Wistar Albino rats. In the present study, twenty-five rats
were used. Five rats were used for the pilot study, and the other twenty were divided
into four equal groups. On day 32 of age, all the animals in Ciprofloxacin/Water and
Ciprofloxacin / Green tea treated groups were subcutaneously injected by ciprofloxacin
as two subcutaneous injections of 600 mg/kg of body weight, eight hours apart, while
the Saline/Water and Saline/Green tea groups were subcutaneously injected by saline.
The Saline/Green tea and Ciprofloxacin / Green tea groups were intragastically gavaged
by green tea extract in an oral dose of 300 mg/kg/day, eight days before the
subcutaneously injection of saline or ciprofloxacin. On day 34, all the animals were
anaesthetized, blood collection by cardiac puncture was taken, and the mandibular
condyle samples were taken immediately and processed. The results showed that the
intragastric gavage of green tea extract can cause a non-significant change in the
magnesium, calcium, and vitamin E serum levels in all the groups studied (p>0.05), but
the zinc serum level in the Saline/Green tea and Ciprofloxacin / Green tea groups
showed a significant increase in comparison with the others two groups (p<0.05). The
Ciprofloxacin/Water group showed a significant decrease in the mandibular condylar cartilage thickness, cartilage cells number, and proteoglycans content in comparison
with the other three groups (p<0.05). Statistical analysis also showed a significant
decrease in Mankin score in the Ciprofloxacin / Green tea group in comparison with the
Ciprofloxacin/Water group (p<0.05). The Ciprofloxacin / Green tea group showed a
significant increase in the Bcl-2 immune expression (p<0.05) and a non-significant
increase (p>0.05) in collagen II immune expression in comparison with the
Ciprofloxacin/Water group. This study provides the first evidence that green tea extract
can decrease the chondrotoxic effects of ciprofloxacin in a rat model.
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