Teng, Khoo@Koo Yan
(2010)
In vivo evaluation of the angiogenic
and antimicrobial properties of
tualang honey using a fvll-tidckness
burn wounds in animal model
in comparison to hydro fibre.
Masters thesis, Universiti Sains Malaysia.
Abstract
Tangential excision and skin grafting are inevitable in managing full-thickness
bums. Wound bed preparations prior to surgery are necessary in order to prevent wound
infection and promote wound healing. Honey can be used to treat bum wounds. However,
not all the honey is the same. The objective of this study is to evaluate the effect on the
wound contraction, antimicrobial properties and histopathological aspects of Tualang
honey in full-thickness bum wounds in a rat model, in comparison to Aquacel dressings.
Thirty-six female Sprague Dawley rats were randomly divided into three groups. Under
anesthesia, three full-thickness bum wounds were created on the dorsum of the rats. The
full-thickness bum wounds were inoculated with a specific organism (104
), namely
Pseudomonas aeruginosa (n=12), Klebsiella pneumoniae {n=l2), or Acinetobacter
baumannii (n=l2). The three bum wounds were dressed with Tualang honey, plain
Aquacel or Aquacel Ag. Swab samples were obtained every 3 days (day 3, 6, 9, 12, 15,
18 and 21) for microbiological analyses. Clinical assessments were performed. At day 7,
14 and 21 days of bum, tissue samples were sectioned and histopathological examination
was performed. The result of this study reveals a rapid 32.26% reduction in wound size
by day 6 (p = 0.008) in the Tualang honey-treated wounds, and 49.27% by day 15 (p =
0.005). The wounds remained smaller by day 18 (p < 0.032). Tualang honey-treated rats
demonstrated a reduction in bacterial growth in Pseudomonas aeruginosa inoculated
wounds (p = 0.005). However, Aquacel-Ag® and plain Aquacel-treated wounds are
superior to honey-treated wounds with Acinetobacter baumannii (p = 0.035). Neutrophil
count was reduced in honey-treated wound on day 7 in both Acinetobacter baumannii and Klebsiella pneumoniae-inoculated wounds (p < 0.05) and similar fmdings on day 14
in Pseudomonas aeruginosa-inoculated wounds (p = 0.003). Granulation thickness of
honey treated wound was 50.42J.Lm compared to Aquacel-treated wound (24.87Jlffi) on
day 14 in Pseudomonas aeruginosa group (p = 0.045). On day 21, in Klebsiella
pneumoniae group, there was complete epidermis coverage of the wound (p = 0.002) and
increased thickness of granulation tissue (p = 0.001) in honey treated group. In histologic
analysis of new capillary fonnation, there was no statistical significance between all the
dressings. In conclusion, this experiment shows the positive effect ofTualang honey as a
topical dressing for full-thickness bum wounds in an animal model. Tualang honey has
better results with regard to the eradication of Pseudomonas aeruginosa. It also promotes
bum wound healing process on wound inoculated with Pseudomonas aeruginosa or
Klebsiella pneumoniae.
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