Rusli, Noradliyanti
(2013)
The effect of crude extract of centella asiatica on voltage-gated sodium
hannel (VGSC) media ted motility on mda-mb-231 breast cancer cell lines.
Masters thesis, Universiti Sains Malaysia.
Abstract
Voltage-gated sodium channel (VGSC) has been found to plays a role in metastasis
as it help in the cancer cells adhesion, motility, secretion as well as invasion. Breast cancer
also shows these properties with neonatal Nav 1.5 to be highly expressed and it is one of the
isoform of the VGSC. This suggests VGSC, specifically neonatal Navl.S to be used as a
target for a new drug development for breast cancer treatment and for anti-metastasis drug.
Cente/la asiatica is one of plant or herb that being used in traditional and alternative
medicine to help cure certain disease or disorder including epilepsy and cognitive disorder
as well as cancer. Thus, in this project, the effect of the Centella asiatica on the voltagegated
sodium channel activities and motility of the MDA-MB-231 breast cancer cell lines
have been studied. The mechanism on how the plant extract shows an effect to metastasis
also being explored by doing a molecular study on the expression of the neonatal Navl.S.
Four different assays are being used in the determination of the effect which includes MIT
assay, Trypan Blue assay, motility assay and molecular study. There is no effect of the
plant extract on the proliferation and viability and toxicity assay but there is an effect on
the motility of the cells. As there is no effect of the plant extract on the cells proliferation
and viability, the effect that can be seen on the motility is genuinely produce by the plant
extract on cell's motility not because of it also affect proliferation and cells viability. From
a molecular study, it has been shown that there is a decrease in the neonatal Nav l.S
expression and this support the fact that the plant extract reduce the migration rate by
decreasing or down-regulating the neonatal Navl.S expression. In conclusion, this plant
crude extract has a great potential to be used in the anti-metastasis drug development
specifically on breast cancer which expressed neonatal Navl.S.
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