Jamil, Noor Izani Noor
(2010)
Construction of indigenous plant extract
library for anti-cancer activity.
Construction of indigenous plant extract library for anti-cancer activity.
Abstract
Barringtonia racemosa, Syzygium polyanthum, Oroxylum indicum and Vitex pinnata
are edible plants commonly consumed as salads and also used in traditional herbal
medicine. These plants were believed to possess anticancer, anti-inflammatory and
anti-diabetic properties, among others. Thus, we seek to determine the antiproliferative
and apoptosis activity of the methanol leaves extracts of these plants on
the growth of cervical cancer cell line, HeLa using methyl1;1ne blue assay. The
methanol leave extract of 0. indicum exerted the strongest antiproliferative activity
towards HeLa cells with IC5o 3.87 jlg/ml without affecting non-malignant cell lines
tested (V ero, MOCK, L929) compared to Cisplatin IC50 of I 0.09 jlg/ml to He La cells.
Barringtonia racemosa, Syzygium polyanthum, and Vitex pinnata showed an IC5o of
8.52, 34.06 and 9.69 jlg/ml on HeLa cells respectively. However, Barringtonia
racemosa and Syzygium polyanthum showed cytotoxic effect on the non-malignant
cell lines. Thus, 0. indicum was selected for nuclear staining by means of Hoechst
staining to access apoptosis events. DNA fragmentation was seen in treated cells and
apoptotic bodies occurred during 24, 48 and 72 hours treatment as was seen in
Cisplatin treatment (positive control drug). Phytochemical screening of 0. indicum
methanol leaves extract indicated the presence of flavonoids, tannins, triterpenes and
steroids which may be responsible for the anti-proliferative and apoptosis activities
towards HeLa cells. The findings suggested that 0. indicum has the selective antiproliferative
activity on HeLa cells by inducing apoptosis.
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