Kannan, T.P.
(2010)
Genotoxic evaluation of locally produced dental porcelain by using:comet assay.
Genotoxic evaluation of locally produced dental porcelain by using: comet assay.
(Submitted)
Abstract
The aim of this study was to determine the genotoxicity of a locally produced dental
porcelain (Universiti Sains Malaysia, Malaysia) using the Comet assay. The protocol for the
Comet assay was followed as per the guidelines proposed by Tice eta!. (2000). L929 (CCL-1
A TCC, USA) mouse fibroblast cells were treated with the dental porcelain at three different
concentrations (50, 100 and 200mg/ml) along with concurrent negative (Eagle's Minimal
Essential Medium) and positive (Zinc sulfate) controls. DNA strand breaks were evaluated
based on the mean tail moment for 150 comets/sample. There was no significant difference
in the tail moment between the negative control and the different concentrations of the
dental porcelain tested, suggesting that the locally produced dental porcelain did not induce
any DNA damage. Also, there was no dose-dependent relationship on the tail moment.
Zinc sulfate, which was used as a positive control induced significant DNA damage. The
results indicate that the locally produced dental porcelain is non-genotoxic under the present
test conditions
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