Rommali, Hafiz
(2011)
DNA Extraction from Blood in Soil.
Masters thesis, Universiti Sains Malaysia.
Abstract
DNA analysis from crime scene samples involve blood that mixed with soil had currently
brought difficulties to forensic scientist due to the presence of inhibitors and also environmental
factors that influenced the quality of the DNA. Murder is the main crime involved in this
situation. Due to difficulties, a simulation of crime scene had been setup for the study. Blood
from different individuals from Blood Transfusion Centre, Universiti Sains Malaysia Hospital
had been poured to the chosen soil location (A and B) and had been left for 28 days for study.
The study aims for determination of DNA concentration from extracted blood on soil in both the
locations; determination of concentration of DNA from soil after a period of time and also soil in
different levels of depth by using Quantifiler kit (Applied Biosystems) using Real-Time PCR.
Environmental factors that had been set to study towards the samples are the pH of soil, humidity
of soil and weather throughout 28 days of study. The results obtained from the study are
unreliable although the Real-Time PCR successfully quantitated the DNA. However, only 6
samples out of 38 samples are able to be quantitated. The remaining samples have failed to be
quantitated due to the presence of natural inhibitors in the soil, and acidic pH of soiL high
humidity of soil due to frequent raining during the period of study. The study should be further
explored for more reliable results by demonstrated multiple extraction methods for removal of
inhibitors in the soil besides optimize the quantity and the quality of the DNA extracts in the
extraction process.
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