Abdul Halim, Shibghatullah
(2008)
The effect of bovine bone scaffold on
the microscopic biological response
of human chondrocytes.
Masters thesis, Universiti Sains Malaysia.
Abstract
Introduction
In the production of cartilaginous tissue, the choice of appropiate scaffold remains as a great
challenge. The currently available scaffold, either natural or synthetic still does not meet the
requirement of a scaffold for cartilage tissue engineering. An ideal scaffold has to provide a
mechanical stability to the individual cell as a construct or transitional framework before
synthesis of new extra cellular matrix.
Objective
The aim of this study was to evaluate bovine bone as a tissue engineering construct for
cartilage reconstruction and to investigate the biological effects of bovine bone on human
chondrocyte in vitro.
Method
Human chondrocytes were cultured and seeded onto bovine bone scaffold with seeding
density of 1 x 1 05 cells per 100 ul I scaffold and incubated for 1 day, 2 days, 5 days and 7
days. Proliferation and viability of the cells were measured by mitochondrial dehydrogenase
activity (MTT assay), adhesion study was analyzed using Scanning Electron Microscopy
(SEM) and differentiation study was analyzed by lmmunoflorescent staining using Confocal
Laser Scanning Electron Microscopy (CLSM).
Result
The data showed the presence of proliferation and viability of the cells on the scaffolds by
MTT method within 24 hours to 7 days observed. SEM pictures revealed presence of
chondrocytes located on the scaffolds, showed increasing number of cell within the days and
that cells readily grew on the surface and into the open pores of the scaffold.
lmmunoflorescent staining detected collagen type II on the scaffolds which was increasing
within the days.
Conclusion
The results showed the potential of bovine bone as three dimensional scaffold for cartilage
tissue engineering because of the good cells proliferation, attachment, maturity, non toxic,
safe, easily resourced and relatively cheap.
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