Badrul Syam, Mat Zainuddin
(2008)
Recombinant salmonella typhi ty21a as
vaccine candidate against
tuberculosis.
Masters thesis, Universiti Sains Malaysia.
Abstract
A recombinant Salmonella Typhi Ty21 a vaccine was developed where the candidate
[rSTvaciii(M2)] displaying a multi-epitopes mycobacterial antigen called the
Vaciii(M2) (containing ubiquitin and 4 immunogenic mycobacterial epitopes of
ESAT-6, PhoSI, Hsp 16.3, and MTB 8.4 genes) on the surface of Salmonella Typhi
Ty21a (Ty2la) using PgsA protein as a carrier. The expression of Vaciii(M2)
protein on the surface of Ty21 a was verified by Western blot. A DNA vaccine was
constructed namely as pVaxVaclll, encoding the Vaclll gene. Immunization in
BALB/c mice with pVaxVaclll and rSTvaciii(M2) either as homologous or
heterologous vaccination, induced strong Th 1-type responses based on high levels of
in vitro IFN-y but low levels of IL-4 secretion. However, the heterologous primeboost
vaccination using pVaxVaclll followed by rSTvaciii(M2) induced lower IFNy
production compared to homologous rSTvaciii(M2) or pVaxVaclll vaccination.
Since the protective immunity against tuberculosis required Th 1-type response, these
rSTvaclii(M2) and pVaxVaclll have potential as effective vaccine candidates
against tuberculosis.
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