Mohd Abdul Halim, Abd Latif
(2009)
Detection of interleukin-lb and tumor necrosis factor (TNF-a) in macrophages treated with recombinant bcg (rBCG)
expressing the 19kDa c-terminus of the merozoite surface protein (MSP-lC).
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
Malaria remains a serious public health problem and ·causes an ·estimated 2 million ·deaths
worldwide each year. The increase of drug resistant parasites and insecticide resistant
mosquito vectors in certain areas worsens the situation especially against P. falciparum. As
such, production ·of vaccine is very important in malaria prevention. In this study, a
recombinant Mycobacterium bovis bacille Calmette-Guerin (rBCG) which expressed the
19kDa C-terminus of the merozoite surface protein (MSP-1 C) was developed against the
blood stage of malaria infection. The inflammatory cytokines ·such as interleukin-1f!(1l-1~)
and tumor necrosis factor-a (TNF-a) produced by macrophages are important for
elimination of malaria infection. The ability of our rBCG construct to stimulate the
production ·of pro-inflammatory cytokines by macrophages was determined at ·different
incubation times (24 hours, 72 hours and 120 hours) and different concentrations (1:20 and
1:40 (macrophage: BCG or rBCG)). This study showed that the production of IL-1P and
TNF--a was -significantly higher in macrophages treated with rBCO compared to BCG,
which could be observed at all incubation times and concentrations performed. This rBCG
construct is able to stimulate the production of cytokine and perhaps preventing malaria
infection especially at the blood stage.
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