Yap, Maxsim Mee Sim
(2006)
Disposable Biomimetic Array Sensor Strip
Coupled With Chemometric Algorithm For
Quality Assessment Of Orthosiphon Stamineus
Benth Samples.
Masters thesis, Perpustakaan Hmzah Sendut.
Abstract
A disposable screen printed array sensor strip based on self-plasticized methacrylate
acrylate PVC blend lipid membranes combined with chemometric algorithm has been
developed and applied for qualitative and quantitative analysis of O.stamineus
samples. For qualitative analysis, the biomimetic array sensor system combined with
Principal Component Analysis (PCA) and Discriminant Analysis (DA) were able to
classify and to verify the geographical origin of the herbal samples. The classification
model built with PCA was useful for the discrimination of the herb according to its parts;
stem and leaves, while was also successfully applicable for different type of
O.stamineus extracts identification. PCA has also been applied for batch to batch
consistency screening of the herb while model built with DA on the other hand was able
to predict the taste of O.stamineus sample which was found to be bitter taste based on
the five basic taste qualities. For quantitative analysis, Partial Least Square (PLS) and
Principal Component Regression (PCR) were applied to the array sensor output to
determine whether the content of sinensetin and caffeic acid in different batches of
O.stamineus extracts could be obtained by these methods. Comparison between the
PLS and PCR models presented in the quantitative analysis showed that PLS have
substantially better predictive capability than PCR and the results obtained were in
good correlation with the results given by the HPTLC method.
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