Hamid, Shahrul Bariyah Sahul
(2011)
Study on the effect of black cumin
Oil and LDL on lipopol ysaccharide
(lps) - induced toll - like receptor
activation in macrophage and
dendritic cell lines.
Study on the effect of black cumin Oil and LDL on lipopol ysaccharide (lps) - induced toll - like receptor activation in macrophage and dendritic cell lines.
Abstract
Lipid-laden macrophage has been reported to play various roles in atherogenesis. The
focus was to elucidate effects of a natural product on the progression of monocytederived
macrophage growth. Nigella saliva was selected as a form of treatment to
macrophage cell growth in culture conditions due to various findings on its medical
benefits. Human monocytes were isolated and grown at 37°C and 5%C02 saturation for
5 days prior to treatment with Nigella sativa oil. The cells were plated and washed before
addition of ox-LDL (lOμg/ml) alone in untreated condition and combination of ox-LDL
(lOμg/ml) and (72 μg/ml) Nigella sativa oil in treated condition. The growth progression
was monitored every 24 hours for 3 days. Nigella sativa oil caused noticeble effect on
macrophage growth compared to monocyte especially 24 hours after treatment. The mean
was significant different between untreated and treated condition for both monocytes and
macrophages (p<0.001). This was shown by the delayed growth pattern as seen in
macrophage compared to monocytes. There was less oil red 0 staining in cells treated
with mixture of oxLDL and Nigella sativa compared to those treated with oxLDL alone.
The signalling of Nigella saliva has been reported to occur via TLR-4 receptor, where it
is significantly present on macrophage cell surface. This may be one of the factors
leading to the growth difference. Hence, progression of macrophage to foam cells could
possibly be controlled with the use of Nigella saliva oil.
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