Wey, Goh Kai
(2008)
The expression of recombinant
bacille calmette-guerin (rBCG) containing
VPI gene of enterovirus 71 (EV71).
Other.
Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia.
(Submitted)
Abstract
Enterovirus 71 (EV71) is a positive-sense single-stranded RNA, nonenveloped,
icosahedrally symmetrical virus which was first detected in 1969 in United States.
EV71 is associated with Hand-Foot-And-Mouth disease (HFMD) and some of EV71
infections may proceed to neurological diseases and even death because of neurogenic
pulmonary edema. To date, there is still no vaccine available against this disease.
Mycobacterium bovis Bacille Calmette-Guerin (BCG) has been suggested to be an
attractive vehicle for the delivery of foreign antigens to the immune system. The main
purpose of this study is to express a recombinant BCG (rBCG) containing ubiquitin
(UbGR)-VP1 fusion gene ofEV71. UbGR-VP1 protein was detected in the cell extract
but not in the supernatant of the recombinant clone by Western blot analysis using rabbit
polyclonal antibody specific to VP 1 protein. The immunogenicity of the rBCG is still
unknown and can only be confirmed if further study is done.
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