Malik, Nurfairuz Abdul
(2021)
Potential of stingless bee honey in modulating skin ageing of human dermal fibroblast cells.
Masters thesis, Universiti Sains Malaysia.
Abstract
Skin is the visible indicator of ageing. During ageing, the excessive
production of reactive oxygen species (ROS) contributes to the increasing of matrix
metalloproteinase (MMP) production that causes collagen degradation. Increasing
formation of ROS can be prevented by antioxidants such as stingless bee honey.
Stingless bee honey is a good source of natural antioxidants that might delay skin
ageing. Therefore, this study aims to elucidate the potential roles of stingless bee
honey on the modulation of skin ageing in human dermal fibroblast cells. The
optimum concentration and incubation time of stingless bee honey were determined
using MTS assay, while lipid peroxidation analysis was quantitated using flow
cytometer. Gene expression of matrix metalloproteinase 1 (MMP-1) and collagen
type I (COL1A1) were analysed using real time RT-PCR. Results showed that the
concentration of stingless bee honey at 0.02% for 72 hours incubation significantly
increased the viability of human dermal fibroblast cells compared to untreated cells.
Treatment with 0.02% of stingless bee honey for 72 hours also showed significant
upregulation of COL1A1 expression in senescent human dermal fibroblast cells and
downregulation of MMP-1 expression in both pre-senescent and senescent human
dermal fibroblast cells. However, no significant changes were observed on lipid
peroxidation analysis in both pre-senescent and senescent human dermal fibroblast
cells when treated with stingless bee honey. In conclusion, this study suggested that
stingless bee honey beneficially delayed skin ageing through downregulation of
MMP-1 expression and potentially promotes collagen synthesis through upregulation
of COL1A1 expression in human dermal fibroblast cells.
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