Omar, Syahirah
(2020)
Comparison of three DNA extraction methods from raw cow's milk sample.
Masters thesis, Universiti Sains Malaysia.
Abstract
The progress and advancement in deoxyribonucleic acid (DNA) extraction
has greatly evolved since it was initally performed in year 1986. DNA extraction has
always be the first step required prior to any DNA analysis. In this study, raw cow’s
milk has been subjected to three different extraction methods for determining the
method that perform best in terms of DNA yield. Genomic DNA extracted from raw
cow’s milk sample by the three methods was evaluated for purity and quantity by
spectrophotometry and gel electrophoresis. On average, all three methods statistically
showed no significant differences on final results of nucleic acid concentration,
however, in terms of cost-effectiveness, conventional methods of phenol-chloroform
and TENS method saved more compared to commercial kit. The results of this study
showed that phenol-chloroform method had the best DNA yield based on the
Nanodrop spectrophotometer measurement, followed by TENS method and
commercial kit.
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