Julia, Abdullah
(2008)
Phytochemical analysis and toxicity test of some selected herbal preparation in market.
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
The phytochemical and the cytotoxicity studies of some selected locally available
herbal preparation on VERO cell lines were done. Three products have been purchased
from the local market namely TAOl, EN02 and AM03. Each product contained mixture of
herbs. For TA01 product, it was claimed to Eurycoma longifolia. Second product, that is
EN02, it was claimed to contain Sauropus androgynus (cekak manis) and for AM03, it was
claim to contain rhizome of Curcuma zedoaria, and the Elephantopus scaber (tapak liman).
Successive extraction of the herbal products TAO 1, EN02 and AM03 yields ( 1.66%, 1.06%
and 3.2% in petroleum ether), (10.6%, 32% and 14.0% in methanol) and (9.26%, 4.65%
and 9.63% in water) respectively. Phytochemical test was done for all the extracts. Every
product was tested positive for the presence of alkaloid, saponin, coumarin, flavonoids,
tannin and phenolic compound except for anthraquinones. Cytotoxicity test was done for
methanol and water for all of the extract. From the cytotoxicity test, all the herbal
preparation has not shown toxic effect on VERO cells. All extracts showed no ICso at
concentration of 1.59 x 10-3
J.1g/ml to O.lJ.Lg/ml.
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