Chang Ching, Ching
(2008)
Cloning and expression of ga protein of
nip ah virus in yeast expression system.
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
Nipah virus (NiV) is a deadly zoonotic paramyxovirus that has emerged and reemerged
over the last 10 years. In human, the infection leads to encephalitis with 92%
mortality rate. This has underlined the importance for the development of a vaccine
candidate in preventing such zoonotic disease. In previous study, the gene encoding the
NiV glycoprotein (NiV -G) was cloned into the yeast Pichia Pastoris expression vector
(pZMF) under the control of AOXI promoter. However, the recombinant G glycoprotein
expression was found to be highly unstable in this system. Therefore, the stability of the
recombinant protein was investigated by studying one of the truncated gene fragment (GA
gene). The truncated gene encoding the gene sequence 1-867 was cloned and expressed in
the same biological system. The detection of the truncated recombinant protein was
performed through Western blot analysis. The result of this study indicated that the
truncated fragment was stable and the expression was consistent with protein molecular
weight of 40 kDa. The absent of degradation incident in this study had suggested that GA
gene fragment was stable in full length G-glycoprotein expression. In addition, the
truncated recombinant protein could be utilized as a promising vaccine candidate if the
recombinant protein was found to be highly antigenic and immunogenic.
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