Jason, Heamaashini Nadarajah
(2019)
Integrated Management In Tissue
Cultured Musa Spp. Against Fusarium
Wilt Disease Tropical Race 4 (Vcg 01213).
PhD thesis, Universiti Sains Malaysia.
Abstract
The economic importance of bananas is expressed in valuable foreign revenue
through export and also constitutes the economic livelihood of rural households
worldwide. The ‘bananageddon’ phenomenon is a term coined to describe the
decimation effect of an infamous fungus Fusarium oxysporum f. sp. cubense (Foc)
Tropical Race 4 (TR4) that plagues the banana industry globally. The aim of this
research was to determine a feasible control strategy in battling Fusarium wilt
disease Tropical Race 4 (VCG 01213) through integrated management. Tissue
culture materials from four cultivars, namely Berangan Intan (AAA), Grande Naine
(AAA), Williams (AAA) and Lemak Manis (AA) were transferred to a Foc hotspot
field to determine somaclonal variation through recurrent resistance selection until
the plants withered and collapsed or until harvest. HYT Chitinase was applied during
planting and also during intervals throughout the growth of the banana plants.
Intercropping of banana tissue culture materials with Allium spp. was field tested as
well as planted inside potted banana materials prior to field planting to confer disease
tolerance. HYT Chitinase was not able to neither induce resistance nor confer
tolerance for the four banana cultivars evaluated at the Foc hot spot area. The
planting of Allium tuberosum inside the potted banana cultivars prior to field planting
did to a certain extent confer tolerance to Berangan Intan cultivar. But, Allium spp.
needs to be established for at least 3 generations in a Foc hot spot area in order to
obtain negligible levels of Fusarium wilt incidences.
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