Mokhtaruddin, Nor Alfarizan
(2006)
Classification of Superbaplogroup M in Chinese
Population of Peninsular Malaysia.
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
The evolution of the human mitochondrial genome is characterized by the
emergence of ethnically distinct lineages or haplogroups. Mitochondrial DNA
(mtDNA) haplogroups were identified on the basis of the presence or absence of a
restriction enzyme polymorphism in the coding region along with the sequence
polymorphisms in the control region. This study was undertaken to analyze the
mtDNA polymorphism in random population of Chinese ethnic of Peninsular
Malaysia. A total number of 44 buccal cells samples were collected and subjected to
extraction, followed by amplification by PCR and digestion process through
restriction fragment length polymorphism (RFLP) analysis. Alu I restriction enzyme
was used to detect polymorphism at nucleotide position 10397 in the mtDNA coding
regions. Result shows that out of 44 samples, 21 of them were identified to be having
an Alu I site gain at nucleotide position 10397, which classified them under the
superhaplogroup M.
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