Khizam, Nur Aizatul Nathasha
(2019)
Development Of Species-Specific
Microsatellite Markers Of Termite
Globitermes Sulphurues Haviland (Blattodea:
Termitidae) For Genetic Population
Analysis By Using Next-Generation
Sequencing (Ngs) Approach.
Masters thesis, Universiti Sains Malaysia.
Abstract
Subterranean termite Globitermes sulphureus (higher termite) comprise a wide
distributed family of termites, found throughout the tropical regions of the world. Little
is known about G. sulphureus role in biology, dispersal range, and their control due to
lack of research to gather molecular data information. Hence, from the availability of
whole sequences of G. sulphureus, a great opportunity to develop species-specific
microsatellite markers can be done. In this study, a total of 243,057 transcripts were
generated from transcriptome dataset of G. sulphureus, with dinucleotide repeats being
the most abundant. From these, a total of new 30 species-specific microsatellite
markers were designed using next-generation sequencing (NGS) procedures. All of 30
markers were successfully amplified and showed visible scorable polymorphism. The
polymorphic information content (PIC) values showed greater than 0.5 in which
proved that all markers here had high level of polymorphism, with 3 to 9 alleles per
locus were identified. The observed and expected heterozygosities ranged from 0 to
0.83 and 0 to 0.98, respectively. The species-specific microsatellites developed in this
study can be used to inferred genetic diversity, breeding pattern and population genetic
structure of G. sulphureus. In addition, the breeding pattern, genetic diversity and
population genetic structure of G. sulphureus remain poorly understood.
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