Phytochemical screening and natural killer cells immunomodulation effects of pereskia bleo leaves extract on cervical cancer cells

Salleh, Siti Farhanah Mohd (2020) Phytochemical screening and natural killer cells immunomodulation effects of pereskia bleo leaves extract on cervical cancer cells. PhD thesis, Universiti Sains Malaysia.

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Abstract

Pereskia bleo is a leafy and edible plant, locally known as 'Pokok Jarum Tujuh Bilah" which has anti-cancer properties. This study purposed to elucidate the underlying mechanism of this plant as anti-cancer in inducing cell death as well as to evaluate its immunostimulatory effects on Natural Killer cells (NK cells) as a potential additional anti-cancer effect. In this study, the leaves of P. bleo were extracted using propenyl) and hexadecanoic acid. PBEA exhibited the lowest IC50 value (14.37 ± 8.40 μg/ml) indicated the strongest cytotoxic effect selectively on cervical cancer cells (HeLa). The cell cycle analysis showed inhibition of cell proliferation at G0/G1 phase in PBEA treated HeLa cells as evidenced by a significant accumulation of the cells at this phase (P<0.05). Morphological examination on PBEA treated HeLa cell showed the presence of fragmented nuclei and condensation of chromatin while apoptosis was detected in the Annexin V/PI assay. Analysis of apoptotic proteins revealed a significant upregulation of pro-apoptotic proteins (Bax, p53 and caspase-3) while downregulation of anti-apoptotic protein Bcl-2 (P<0.05) in PBEA treated HeLa cells. Meanwhile, NK cells proliferation at 24 h was found significantly increased compared to 48 h and 72 h of PBEA treatment (P<0.05). Apoptosis of HeLa cells was markedly increased in PBEA treated NK cells from cancer patients. This extract also enhanced granzyme B and IFN-

Item Type: Thesis (PhD)
Uncontrolled Keywords: anti-cancer
Subjects: R Medicine
Divisions: Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Perubatan (School of Medical Sciences) > Thesis
Depositing User: Mr Abdul Hadi Mohammad
Date Deposited: 21 Dec 2020 08:33
Last Modified: 21 Dec 2020 08:39
URI: http://eprints.usm.my/id/eprint/48014

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