Abdullah, Nur Azeelah
(2018)
Genetic variations of six orang asli sub-groups in Peninsular Malaysia using autosomal and x-chromosome short tandem repeats (STRs) analysis.
Masters thesis, Universiti Sains Malaysia.
Abstract
The Orang Asli population represent only about 0.5% of total population of
Malaysia. There are 18 sub-groups of Orang Asli that belong to the Semang, Senoi
and Proto-Malay. These sub-groups have diverse cultures, traditions, beliefs and
languages depending on their ethnicity and location. In this study, a total of 164 blood
samples from six Orang Asli sub-groups in Peninsular Malaysia; Semai, Che Wong,
Orang Kanaq, Lanoh, Bateq and Kensiu were PCR-type for 15 autosomal STR loci
using Identifiler Direct system and 12 X-chromosomal STR loci using Argus X-12
QS system. The allele frequencies and several forensic parameters for STR as well as
the genetic structure in the Orang Asli sub-groups were determined. The agreement
with Hardy-weinberg Equilibrium (HWE) was confirmed for all loci in Semai and
Lanoh sub-groups for autosomal STR results and Che Wong for X-STR results. The
combined power of exclusion (CPE) were higher than 0.999. The combined power of
discrimination (CPD) also supports the theory that no two individuals would have the
same genotype except for identical twins. FGA is the most variable and informative
marker for forensic testing of the 15 autosomal STR loci while DXS10146 is the most
informative marker for X-STR system of the Orang Asli in Peninsular Malaysia. The
Orang Asli sub-groups were plotted closed to each other in the Principal Component
Analysis (PCA), suggesting some degree of common ancestry between the
subgroups.These findings indicate the uniqueness of the Orang Asli sub-groups and
the evidence of their ancient genetic affinities as they represent the first human
migration wave out of Africa. The STR and X-STR profile dataset are important for
forensic applications and for establishment of the Orang Asli population data in
Peninsular Malaysia, which are currently not available for this population.
Actions (login required)
 |
View Item |
