Muhammad, Nur Amalina
(2018)
The effect of diet and BMI on gut microbiota profile among primary school children in Kota Bharu, Kelantan.
Masters thesis, Universiti Sains Malaysia.
Abstract
Human gut microbes are present in large concentration on intestinal mucosal surfaces and
play important roles in host health. Obesity and dietary intake are known to have implications
in driving factors for structure of gut microbiota.
Method
The present study examined the composition of the gut microbial community among primary
school children in Kota Bharu, aged 7-11 years old (n=81). Fecal sample were collected and
subjected to 16S rRNA gene sequencing analysis. Dietary survey were obtained in order to
assessed the association of diet between gut microbiota.
Result
Analysis of bacterial composition according to taxonomic rank (genus, family and phylum)
revealed most abundance of bacterial at genus level were Bacteroides 23% and Prevotella 22
% respectively. The microbiota were classified into two enterotype like clusters , each driven
by Bacteroides (B-Type) or Prevotella (P-Type). Statistical analysis revealed B-Type and PType
shows significant association with BMI (p value =0.005).We also found that B-Type of
microbiota positively associated with chicken and fish (p value = 0.007 and 0.038
respectively), whereas P-Type showed positively associated with fruit, milk & dairy product,
seafood, seasoning & flavourings and beverage (p value = 0.025, 0.020, 0.032, 0.001 and
0.012 respectively).
Conclusion
There was an association between BMI and diet on gut microbiota among healthy primary
school children in the Kota Bharu population. Further studies are necessary to elucidate the
mechanism behind these changes and ultimately their link to health and disease.
Actions (login required)
 |
View Item |
