Witjaksono, Widowati and Ab Rani, Samsudin and Naing@Sadiq, Lin@Mohd Ayub
(2004)
Sealing ability of hydroxyapatite as a root canal sealer: in vitro study.
Project Report.
Pusat Pengajian Sains Pergigian, Universiti Sains Malaysia.
(Submitted)
Abstract
occupied by pulp tissue.Gutta-percha is used with various techniques for obturation of the root
canal system, and If combine- with lateral condensation remain the most widely accepted and used
obturation technique.The most common cause of failure Involving endodontic treatment can be
attributed to the lack of an apical seal leading to leakage at the apex. Hydroxyapatite (HA) is the
most thermodynamically synthetic calcium phosphate ceramic, and has indicated useful as a sealer
because can seal a furcation perforation, Is shown to be biocompatible and also has potential to
promote the healing of bone In endodontic therapy. The objective of this study is to detennlne the
sealing ability of HA produced by School of Engineering, Unlversiti Sains Malaysia (USM) when
used as a sealer in root canal obturation. compare with Tubll-seal ( Zinc-Oxide base ) and Sealapax
( Caldum Hydroxyde base ) sealers. ~
Forty five single rooted human extracted anterior teeth were instrumented and randomly divided
Into three experimental groups of 15 teeth each. All teeth in the experimental groups were
obturated with later$11y condensed gutta percha technique. Teeth In the first group were sealed
using Zinc-Oxide (ZnO) based sealer and those of second group using Calcium Hydroxide (CaOH)
based root canal sealer. Third experimental group was sealed using HA from School of
Engineering,USM. Teeth were then suspended in 2% methylene blue. After this, teeth were
demineralized dehydrated and cleared. Linear dye penetration was detennined under magnifying
tense with calibrated eye piece.
Statistical analyses of the linear dye pene~tion were performed with Kruskal Wallis test. hJ for the
Inter group comparison between HA and ZnO groups and between HA and C&OH groups were
analysed by Mann-Whitney test.
The resuHs showed that dye penetration for group which were sealed with HA exhibited the lowest
penetration and It showed that there was a statistically significant difference both between HA and
ZnO groups and also between HA and CaOH groups (p<0.001).
We conclude that value added HA based endodontic material which were produced by USM can be
used as a root canal sealing materials when It used In combination with epoxy resin since it leaked
comparatively less as compared to ZnO and CaOH sealers. Before reaching a definitive
conclusion. this material requires further extensive exploration both clinically and In vitro .
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