Zaabar, Wan Razlin Wan
(2011)
Development Of Fluorescing Methicillin Resistant Staphylococcus Aureus (Mrsa) As A Tool For The Screening Of Antibacterial Properties Of Mimusops Elengi Linn.
Masters thesis, Universiti Sains Malaysia.
Abstract
Staphylococcus aureus is a Gram positive bacterium that can cause abscesses,
various pyogenic infections (e.g. endocarditis and osteomyelitis), food poisoning and
toxic-shock syndrome. It is also one of the most common causes of nosocomial
infection (pneumoniae, septicaemia and surgical-wound infections). The Methicillin
Resistant Staphylococcus aureus (MRSA) is a strain that is resistant to β-lactam
antibiotic by virtue of changes in the penicillin-binding protein within their cell
membrane. Consequently, all antibiotics that has β-lactam ring like penicillin group
and cephalosporin group are unable to inhibit the growth of this organism.
Green fluorescent protein (GFP) is a protein of unknown function found in
the jellyfish, Aequorea victoria. This GFP-fusion protein shows a punctuate pattern
when localizes in the cytoplasm. The present study focused on the development of a
fluorescing MRSA by the construction of MRSA vector carrying GFP gene with the
intention of determining whether the fluorescing MRSA strain can be used as a tool
for the rapid screening of antibacterial properties of natural product. The natural
products that had been used were aqueous and several organic solvents extracts from
the bark of plant Mimusops elengi Linn. known locally as ‘Bunga Tanjung’ plant.
This plant has been reported to contain a potent antibacterial component. In this
study, phytochemical investigation of aqueous, ethanolic and ethyl acetate extracts of M. elengi Linn. revealed the presence of alkaloids, flavonoids and tannin
compounds. Whereas, the diethyl ether and petroleum ether extracts revealed the
presence of alkaloids only but absence of flavonoids and tannin compounds. These
secondary metabolites are known to be synthesized in response to microbial
infection.
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