Wahab, Nor Akmal
(2004)
Association of 8-isoprostanes level and renal impairment in
spontaneously hypertensive rats.
Association of 8-isoprostanes level and renal impairment in spontaneously hypertensive rats.
(Submitted)
Abstract
Prolonged uncontroled hypertension is known to cause renal impairment .
The precise mechanism by which raised systemic blood pressure leads to renal
impairment is unclear. Free radicals have been implicated in cell damage and
hypert~nsive has been shown to have higher level of plasma lipid peroxidation
end product malondialdehyde (N.IDA) than normal individuals . This study
· aims to ascertain the levels ofMDA in spontaneously hyp,ertensive rats (SHR)
and determine the age at which changes in the levels ofMDA and renal
impairment begin to occur in SHR.
Sixty-six r;nale SHR, aged 8 weeks~- were divided into eleven groups. A similar
number of male Wistar Kyoto rats (WKY) were also similarly divided to act as
parallel controls. Body weight , systemic blood pressure and plasma ~A were
measured every four \¥eeks starting from the age of 8th weeks until the age of
48th week. Plasma MDA was measured using High Performance Liquid
Chromatography (HPLC). Every four weeks, six rats per group were
anaesthetized for measurement of Glomerular Filtration Rate (GFR), using
standard clearance procedures, to assess the renal function.
Mean body weight of SHR was consistently lower than that of WKY rats from
the·age of 12 weeks onwards (p < 0.05). Blood pressure, on the other hand, was
significantly higher in the SHR when compared to that in age-matched WKY rats
from the age of8 weeks onwards (p < 0.05). Interestingly, plasma :MDA levels
was higher in the SHR than in the controls only from the age of 12 weeks,
reaching statistical significance by the age of24, 44 and 48 weeks. GFR was
lower iii 'SFIR from The age of24 weeks onwards (p .. <0.05). It appears that there
is an age-related decline in GFR in SHR, implying renal impairment , particularly
from the age of 24 weeks onwards when compared to WKY rats but plasma :MD A
levels was higher in the SHR than in the controls from the age of 12 weeks. It is
unclear if the decrease in GFR is a result of the raised levels ofMDA, but it
appears that renal impairment occurs subsequent to changes in plasma :MDA
levels. Clearly, more studies are needed to ascertain the relationship between the
changes in GFR and :MDA in hypertension.
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