Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate

Ab Ghani, Balqis (2017) Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate. PhD thesis, Universiti Sains Malaysia.

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Abstract

Polyhydroxyalkanoate (PHA) is a family of polyesters produced by bacteria that possesses physical properties for various industrial applications. In this study, PHA synthase (PhaC) gene isolated from Chromobacterium sp. USM2 (phaCCs USM2) was explored for its potential to synthesize polymer in Cupriavidus necator. A broad-host range plasmid pBBR1MCS-2 was modified to construct an expression plasmid pYEB-100, which was designed to facilitate cloning of PHA synthases in order to assess the performance of various PhaCs and the composition of PHA accumulated in a standard environment.

Item Type: Thesis (PhD)
Subjects: Q Science > QH Natural history > QH1 Natural history (General - Including nature conservation, geographical distribution)
Divisions: Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Thesis
Depositing User: ASM Ab Shukor Mustapa
Date Deposited: 10 Sep 2019 02:45
Last Modified: 10 Sep 2019 02:45
URI: http://eprints.usm.my/id/eprint/45355

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